Abstract
The development of single cell RNA sequencing (scRNA-seq) has enabled innovative approaches to investigating mRNA abundances. In our study, we are interested in extracting the systematic patterns of scRNA-seq data in an unsupervised manner, thus we have developed two extensions of robust principal component analysis (RPCA). First, we present a truncated version of RPCA (tRPCA), that is much faster and memory efficient. Second, we introduce a noise reduction in tRPCA with \(L_2\) regularization (tRPCAL2). Unlike RPCA that only considers a low-rank L and sparse S matrices, the proposed method can also extract a noise E matrix inherent in modern genomic data. We demonstrate its usefulness by applying our methods on the peripheral blood mononuclear cell (PBMC) scRNA-seq data. Particularly, the clustering of a low-rank L matrix showcases better classification of unlabeled single cells. Overall, the proposed variants are well-suited for high-dimensional and noisy data that are routinely generated in genomics.
K. Gogolewski and M. Sykulski—These authors equally contributed to this work.
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Acknowledgements
This work was supported by the Polish National Science Centre grant no. 2016/21/N/ST6/01507 and no. 2016/23/D/ST6/03613. The authors thank B. Miasojedow, Ph.D. for comments and suggestions.
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Gogolewski, K., Sykulski, M., Chung, N.C., Gambin, A. (2018). Truncated Robust Principal Component Analysis and Noise Reduction for Single Cell RNA-seq Data. In: Zhang, F., Cai, Z., Skums, P., Zhang, S. (eds) Bioinformatics Research and Applications. ISBRA 2018. Lecture Notes in Computer Science(), vol 10847. Springer, Cham. https://doi.org/10.1007/978-3-319-94968-0_32
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