Abstract
Random Amplified Polymorphism DNA (RAPD) analysis is a well-known method for studying genetic relationships between individuals. In this context, processing the underlying RAPD images is a quite difficult problem, affected by various factors. A method for processing RAPD images is proposed, which aims to detect bands by pre-processing the images by performing a template correction step and a band detection mechanism. The results on RAPD images, which aim to verify genetic identity among tree individuals, show that the proposed method detects either negative or positive amplification (bands) with a sensitivity of over 94%.
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References
Antoniol, G., Ceccarelli, M.: A Markov Random Field Approach to Microarray Image Gridding. In: Proc. of the 17th International Conference on Pattern Recognition, Cambridge, UK, pp. 550–553 (2004)
De Boor, C.: A Practical Guide to Splines. Springer, Heidelberg (2001)
Burger, W.: Digital Image Processing with Java and ImageJ. Springer, Heidelberg (2006)
Cao, W., Scoles, G., Hucl, P., Chibbar, R.: Phylogenetic Relationships of Five Morphological Groups of Hexaploid Wheat Based on RAPD Analysis. Genome 43, 724–727 (2000)
Casasoli, M., Mattioni, C., Cherubini, M., Villani, F.: A Genetic Linkage Map of European Chestnut (Castanea Sativa Mill.) Nased on RAPD, ISSR and Isozyme Markers. Theoretical Applied Genetics 102, 1190–1199 (2001)
Das, R., Laederach, A.: GelQuant User’s Manual. Stanford University, Electronically (2004), available at http://safa.stanford.edu/release/GelQuantGuiv09b3UserGuide.pdf
Doyle, J., Doyle, J.: Aislation of Plant DNA from Fresh Tissue. Focus 12, 13–15 (1990)
Fernandez, M., Valenzuela, S., Balocchi, C.: RAPDs and Freezing Resistance in Eucalyptus Globulus. Electronic Journal of Biotechnology 9, 303–309 (2006)
Groos, C., Gay, G., Perrenant, M., Gervais, L., Bernard, M., Dedryver, F., Charmet, G.: Study of the Relationship Between Pre-harvest Sprouting and Grain Color by Quantitative Trait Loci Analysis in the White x Red Grain Bread-wheat Cross. Theoretical Applied Genetics 104, 39–47 (2002)
Helgason, S.: The Radon Transform, 2nd edn. Springer, Heidelberg (1999)
Herrera, R., Cares, V., Wilkinson, M., Caligarip, D.: Characterisation of Genetic Variation between Vitis Vinifera Cultivars from Central Chile Using RAPD and Inter Simple Sequence Repeat Markers. Euphytica 124, 139–145 (2002)
Bio Rad Laboratories: Quantity One User’s Guide. Bio-Rad Laboratories (2000), Electronically available at http://www.calpoly.edu/~bio/ubl/equip_files/q1_manual.pdf
Narváez, C., Valenzuela, J., Mu noz, C.: Comparación de RAPD Y AFLP Como Métodos de Identificación Genética de Vid Basados en el Estudio de Fragmentos Genómicos Anónimos. Agricultura Técnica 60, 320–340 (2000)
Nkongolo, K.: RAPD Variations Among Pure and Hybrid Population of Picea Mariana, P. rubens, and P. glauca, and Cytogenetic Stability of Picea hybrids: Identification of Species-specific RAPDs Markers. Plants Systems Evolution 215, 229–293 (1999)
Rueda, L., Uyarte, O., Valenzuela, S., Rodriguez, J.: Band Detection in Random Amplified Polymorphism DNA Images. Technical Report, Department of Computer Science, University of Concepción (2007), Electronically available at http://www.inf.udec.cl/~lrueda/papers/BandDetectJnl.pdf
Saal, B., Struss, D.: RGA-and RAPD-derived SCAR Markers for a Brassica B-genome Introgression Conferring Resistance to Blackleg in Oilseed Rape. Theoretical Applied Genetics 111, 281–290 (2005)
Sudapak, M., Akkaya, M., Kence, A.: Analysis of Genetics Relationships Among Perennial and Annual Cicer Species Growing in Turkey Using RAPD Markers. Theoretical Applied Genetics 105, 1220–1228 (2002)
Tripathi, S., Mathish, N., Gurumurthi, K.: Use of Genetic Markers in the Management of Micropropagated Eucalyptus Germplasm. New Forests 31, 361–372 (2006)
Williams, J., Kubelik, A., Livak, K., Rafalski, J., Tingey, S.: DNA Polymorphism Amplified by Arbitrary Primers Useful as Genetic Markers. Nucleic Acid Research 18, 6531–6535 (1990)
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Rueda, L., Uyarte, O., Valenzuela, S., Rodriguez, J. (2007). Processing Random Amplified Polymorphysm DNA Images Using the Radon Transform and Mathematical Morphology. In: Kamel, M., Campilho, A. (eds) Image Analysis and Recognition. ICIAR 2007. Lecture Notes in Computer Science, vol 4633. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-74260-9_95
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DOI: https://doi.org/10.1007/978-3-540-74260-9_95
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-74258-6
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