Abstract
Quantum dot fluorophores provide a photo and bio-stable optical marker signal well suited to the tracking of lineage within large cell populations over multiple generations. We have used a Monte Carlo algorithm to model the process of dot partitioning and dilution by cell mitosis. A Genetic Algorithm was used to compare simulated and experiment quantum dot distributions, which shows that the dot fluorescence is divided with a stochastic variation about an asymmetric mean split ratio.
This is a preview of subscription content, log in via an institution to check access.
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Dumitriu, I.E., Mohr, W., Kolowos, W., Kern, P., Kalden, J.R., Herrmann, M.: 5,6-Carboxyfluorescein Diacetate Succinimidyl Ester-Labeled apoptotic and necrotic as well as detergent-treated cells can be traced in composite cell samples. Analytical Biochemistry 299, 247–252 (2001)
Hasbold, J., Hodgkin, P.D.: Flow cytometric cell division tracking using nuclei. Cytometry 40, 230–237 (2000)
Bernard, S., Pujo-Menjouet, L., Mackey, M.C.: Analysis of cell kinetics using a cell division marker: Mathematical modeling of experimental data. Biophysical Journal 84, 3414–3424 (2003)
Alivisatos, A.P.: Semiconductor clusters, nanocrystals, and quantum dots. Science 271, 933–937 (1996)
Pinaud, F., Michalet, X., Bentolila, L.A., Tsay, J.M., Doose, S., Li, J.J., Iyer, G., Weiss, S.: Advances in fluorescence imaging with quantum dot bio-probes. Biomaterials 27, 1679–1687 (2006)
Jyoti, K., Sanford, M.S.: Potentials and pitfalls of fluorescent quantum dots for biological imaging. Trends in Cell Biology 14, 497–504 (2005)
Hines, M.A., Guyot-Sionnest, P.: Synthesis and characterization of strongly luminescing ZnS-Capped CdSe nanocrystals. J. Phys. Chem. 100, 468–471 (1996)
Dabbousi, B.O., Rodriguez-Viejo, J., Mikulec, F.V., Heine, J.R., Mattoussi, H., Ober, R., Jensen, K.F., Bawendi, M.G. (CdSe) ZnS core-shell quantum dots: synthesis and characterization of a size series of highly luminescent nanocrystallites. J. Phys. Chem. 101, 9463–9475 (1997)
Marquez, N., Chappell, S.C., Sansom, O.J., Clarke, A.R., Court, J., Errington, R.J., Smith, P.J.: Single cell tracking reveals that Msh2 is a key component of an early-acting DNA damage-activated G2 checkpoint. Oncogen 22, 7642–7648 (2003)
Errington, R.J., Ameer-Beg, S.M., Vojnovic, B., Patterson, L.H., Zloh, M., Smith, P.J.: Advanced microscopy solutions for monitoring the kinetics and dynamics of drug-DNA targeting in living cells. Adv. Drug. Deliv. Rev. 57, 153–167 (2005)
Vose, M.D.: The Simple Genetic Algorithm: Foundations and Theory, 1st edn. MIT Press, London (1999)
Njoh, K.L., Summers, H.D., Errington, R.J., Brown, M.R., Chappell, S.C., Rees, P., Matthews, D.R., Wiltshire, M., Smith, P.J.: Analysis of cell cycle perturbation using Quantum Dot fluorescence, July 2007, To be submitted to Journal of Biophysics (in preparation)
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 2007 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Brown, M.R. et al. (2007). Computational Simulation of Optical Tracking of Cell Populations Using Quantum Dot Fluorophores. In: Calder, M., Gilmore, S. (eds) Computational Methods in Systems Biology. CMSB 2007. Lecture Notes in Computer Science(), vol 4695. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-75140-3_7
Download citation
DOI: https://doi.org/10.1007/978-3-540-75140-3_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-75139-7
Online ISBN: 978-3-540-75140-3
eBook Packages: Computer ScienceComputer Science (R0)