Skip to main content

Plant Phenotyping with Low Cost Digital Cameras and Image Analytics

  • Conference paper
  • First Online:
Information Technologies in Environmental Engineering

Part of the book series: Environmental Science and Engineering ((ENVENG))

Abstract

In this paper we discuss a prototype, easy-to-deploy, and low cost (∼ $250) phenotype collection system for growth chambers. Off the shelf digital cameras, wireless transmitters, and PCs are used to store and process the images. A Matlab pipeline is used to fuse multiple images, identify the location of each Arabidopsis plant, segment its leaves, and measure leaf topology and area. Our early findings (unpublished) using this system for correlating genotype to phenotype are very promising. We hope that with future improvements and broad adoption, it will have the same disruptive effects as the first “build your own” microarrayers, which allowed for the explosion of genotyping information. Low cost genotyping and phenotyping will hopefully address some of the environmental, agricultural, and industrial sustainability challenges we are facing today.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Nathan P. Gillett, Dáithí A. Stone, Peter A. Stott, Toru Nozawa, Alexey Yu. Karpechko1, Gabriele C. Hegerl, Michael F. Wehner & Philip D. Jones (2008). Attribution of polar warming to human influence. Nature Geoscience. 1. 750-754

    Article  Google Scholar 

  • Pierre Crosson (1997). Impacts of Climate Change on Agriculture. Climate Issues Brief 4.

    Google Scholar 

  • Pittock, B. (2003).Climate change: An Australian Guide to the Science and Potential Impacts, Australian Greenhouse Office.

    Google Scholar 

  • Granier, et al., (2005). PHENOPSIS, an automated platform for reproducible phenotyping of plant responses to soil water deficit in Arabidopsis thaliana permitted the identification of an accession with low sensitivity to soil water deficit. New Phytologist. 169(3), 623-635.

    Article  Google Scholar 

  • Hilbert, D.W., Ostendorf, B. and Hopkins, M., (2001).Sensitivity of tropical forests to climate change in the humid tropics of North Queensland. Austral Ecology. 26, 590–603.

    Article  Google Scholar 

  • William E. McClain (1997). Prairie establishment and landscaping. Nature Heritage Technical Publication.

    Google Scholar 

  • Burel, F. and Baudry, J., (1995). Species biodiversity in changing agricultural landscapes: A case study in the Pays d’Auge France. Agric. Ecosyst. Environ. 55, pp. 193–200.

    Article  Google Scholar 

  • Edwards, D. and Batley, J. (2004). Plant bioinformatics: from genome to phenome. Trends in Biotechnology, 22(5):232-237.

    Article  CAS  Google Scholar 

  • Lussier, Y. A. and Liu, Y. (2007). Computational approaches to phenotyping: high-throughput phenomics. Proc Am Thorac Soc, 4(1):18-25.

    Article  Google Scholar 

  • Aurelie Bonin (2008). Population Genomics:a new generation of genome scans to bridge the gap with functional genomics. Molecular ecology. 17, 3583 – 3584.

    Article  Google Scholar 

  • Nosil P, Egan, SR, Funk DJ (2008). Heterogeneous genomic differentiation between walking-stick ecotypes: ’isolation by adaptation’ and multiple roles for divergent selection. Evolution.62,316-336.

    Article  Google Scholar 

  • Boyes, D. C., Zayed, A. M., Ascenzi, R., Mccaskill, A. J., Hoffman, N. E., Davis, K. R., and Gorlach, J. (2001). Growth stage-based phenotypic analysis of arabidopsis: A model for high throughput functional genomics in plants. Plant Cell.13(7):1499-1510.

    Article  CAS  Google Scholar 

  • Tim Anderson (2009). How to Make Your Own Waterproof Camera Enclosure. Online [http://web.media.mit.edu/~tim/pix/waterproofcamera.html]

  • Ronald J. Hause (2008). Deleting images with interval shooting. Online. [http://chdk.setepontos.com/index.php/topic,2003.0.html]

  • Maik, V., Cho, D., Shin, J., and Paik, J. (2007). Regularized restoration using image fusion for digital auto-focusing. Circuits and Systems for Video Technology, IEEE Transactions on. 17(10):1360-1369.

    Article  Google Scholar 

  • Burt, P. J. and Kolczynski, R. J. (1993). Enhanced image capture through fusion. Computer Vision, 1993. Proceedings., Fourth International Conference on. pp 173-182.

    Google Scholar 

  • De, I. and Chanda, B. (2006). A simple and efficient algorithm for multifocus image fusion using morphological wavelets. Signal Process. 86(5):924-936.

    Article  Google Scholar 

  • Blum, R. (2005). Robust image fusion using a statistical signal processing approach. Information Fusion. 6(2):119-128.

    Article  Google Scholar 

  • Kim, S. K. and Paik, J. K. (1998). Out-of-focus blur estimation and restoration for digital auto-focusing system. Electronics Letters. 34(12):1217-1219.

    Article  Google Scholar 

  • Wu, X. (1992). Color quantization by dynamic programming and principal analysis. ACM Trans. Graph. 11(4):348-372.

    Article  Google Scholar 

  • Wikipedia contributors (2008) YIQ. Wikipedia, The Free Encyclopedia. Online http://en.wikipedia.org/w/index.php?title=YIQ&oldid=259429685

  • Li, C., Xu, C., Gui, C., and Fox, M. D. (2005). Level set evolution without re-initialization: a new variational formulation. CVPR 2005. IEEE Computer Society Conference on. 1: 430 – 436.

    Google Scholar 

  • Fitzgibbon, A., Pilu, M., and Fisher, R. B. (1999). Direct least square fitting of ellipses. Pattern Analysis and Machine Intelligence, IEEE Transactions on. 21(5):476-480.

    Article  Google Scholar 

  • Mai, F., Hung, Y., Zhong, H., and Sze, W. (2008). A hierarchical approach for fast and robust ellipse extraction. Pattern Recognition. 41(8):2512-2524.

    Article  Google Scholar 

  • Mokhtarian, F. and Abbasi, S. (2004). Matching shapes with self-intersections:application to leaf classification. Image Processing, IEEE Transactions on. 13(5):653-661.

    Article  Google Scholar 

  • Parvin, B., Yang, Q., Fontenay, G., and Barcellos-Hoff, M. H. (2002). Biosig: an imaging bioinformatic system for studying phenomics. Computer. 35(7):65-71.

    Article  Google Scholar 

  • DeRisi, J., Iyer, V, and Brown, P.O. (1998), The MGuide: A Complete Guide to Building Your Own Microarrayer. Stanford, CA, Stanford University 1998.

    Google Scholar 

Download references

Acknowledgements

We would like to thank Prof. Borevitz (Department of Ecology and Evolution at University of Chicago) for providing us with the images and initial funding for this effort. Finally, we should thank Ron Hause, a graduate student from the Committee on Genetics, Genomics, and Systems Biology who did his rotation in Prof. Borevitz’s Lab, for his assistance in the development of this project.

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2009 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Tsaftaris, S.A., Noutsos, C. (2009). Plant Phenotyping with Low Cost Digital Cameras and Image Analytics. In: Athanasiadis, I.N., Rizzoli, A.E., Mitkas, P.A., Gómez, J.M. (eds) Information Technologies in Environmental Engineering. Environmental Science and Engineering(). Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-88351-7_18

Download citation

Publish with us

Policies and ethics