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Numerical and Experimental Analysis of the p53-mdm2 Regulatory Pathway

  • Conference paper
Digital Ecosystems (OPAALS 2010)

Abstract

The p53 tumour suppressor plays key regulatory roles in various fundamental biological processes, including development, ageing and cell differentiation. It is therefore known as “the guardian of the genome” and is currently the most extensively studied protein worldwide. Besides members of the biomedical community, who view p53 as a promising target for novel anti-cancer therapies, the complex network of protein interactions modulating p53’s activity has captivated the attention of theoreticians and modellers due to the possible occurrence of oscillations in protein levels in response to stress. This paper presents new insights into the behaviour of the p53 network, which we acquired by combining mathematical and experimental techniques. Notably, our data raises the question of whether the discrete p53 pulses in single cells, observed using fluorescent labelling, could in fact be an artefact. Furthermore, we propose a new model for the p53 pathway that is amenable to analysis by computational methods developed within the OPAALS project.

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Authors and Affiliations

  1. Surgery and Oncology, Ninewells Hospital, University of Dundee, DD1 9SY, Dundee, UK

    Ingeborg M. M. van Leeuwen, Ian Sanders, Oliver Staples, Sonia Lain & Alastair J. Munro

  2. Microbiology, Tumor and Cell Biology, Karolinskat Institute, SE-17177, Stockholm, Sweden

    Ingeborg M. M. van Leeuwen & Sonia Lain

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  1. Ingeborg M. M. van Leeuwen
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  3. Oliver Staples
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  1. Instituto de Pesquisas em Tecnologia e Inovacao (IPTI), Av. Sao Luis, 86, cj 192-19 Andar, CEP 01046 000, Sao Paulo, Brazil

    Fernando Antonio Basile Colugnati , Lia Carrari Rodrigues Lopes  & Saulo Faria Almeida Barretto ,  & 

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van Leeuwen, I.M.M., Sanders, I., Staples, O., Lain, S., Munro, A.J. (2010). Numerical and Experimental Analysis of the p53-mdm2 Regulatory Pathway. In: Antonio Basile Colugnati, F., Lopes, L.C.R., Barretto, S.F.A. (eds) Digital Ecosystems. OPAALS 2010. Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering, vol 67. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-14859-0_20

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  • DOI: https://doi.org/10.1007/978-3-642-14859-0_20

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