Abstract
A method was described for the acute isolation of neurons suitable for patch-clamping study from the frontal cortex of 7-10-d-old Kunming mice by a combination of mechanical and enzymatic means. Using inverted microscope and whole-cell configuration of patch-clamp technique, the morphological and electrophysiological properties of cortical neurons were studied respectively. It was shown that the enzymatically isolated neurons had plump profile, smooth surface, strong aureole and long survival time, met the electrophysiological requirements, and exhibited the whole-cell transmembrane currents, voltage-gated sodium and potassium currents. The experiment proves that this method is simple, efficient, reliable and utility. The dissociated cortical neurons could be obtained and applied to patch-clamping study, which has reference value for studying the effects of physiology, pathology, pharmacology and physical factors on the ion channels of the cortical neurons of mice.
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Li, Yy., Cheng, Lj., Li, G., Lin, L., Li, Dd. (2010). Acute Isolation of Neurons Suitable for Patch-Clamping Study from Frontal Cortex of Mice. In: Li, K., Jia, L., Sun, X., Fei, M., Irwin, G.W. (eds) Life System Modeling and Intelligent Computing. ICSEE LSMS 2010 2010. Lecture Notes in Computer Science(), vol 6330. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-15615-1_72
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DOI: https://doi.org/10.1007/978-3-642-15615-1_72
Publisher Name: Springer, Berlin, Heidelberg
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