Abstract
In this paper, we developed the utilization of excess activated sludge (EAS) to extract protein. Firstly, activated sludge was hydrolyzed, using six different commercial proteolytic enzymes by the Kjeldahl method, at their optimal conditions respectively, and Alcalase was chosen as the ideal for further analysis. Secondly, important parameters on protein extraction efficiency(PEE) of EAS by hydrolysis were investigated. Experimental results have been shown that 52.5% of protein was extracted from EAS (accounting for 30.5% of initial dry sludge (DS) weight) and reducing the quality of activated sludge about 35% under optimum hydrolysis conditions. This novel technology is more environmentally friendly than the conventional sludge treatments.
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References
Wei, Y.J., Liu, Y.S.: Effects of sewage sludge compost application on crops and cropland in a 3-year field study. J. Chemosphere 59, 1257–1265 (2005)
McCarty, P.: Sludge concentration-needs, accomplishments and future goals. J. Water Pollut Control Fed. 38, 493 (1966)
Chudoba, P., Capdeville, B.: A possible way towards reduction of waste sludge production. In: Sixth IAWPRC Conference on Design and Operation of Large Wastewater Treatment Plants, Prague (1991)
Bagreev, A., Bashkova, S., Locke, D.C., Bandosz, T.J.: Sewage sludge-derived materials as efficient adsorbents for removal of hydrogen sulfide. J. Environ. Sci. Technol. 35, 1537–1543 (2001)
Ministry of Health of PRC, GB 5009.5-2010. Determination of protein in foods. Standards Press of China, Beijing (2010)
Crooke, W.M., Simpson, W.E.: Determination of ammonium in Kjeldahl digests of crops by an automated procedure. J. Agric. Food Chem. 27, 1256–1262 (1971)
Tanaka, S., Kobayashi, T., Kamiyama, K., Bildan, M.N.: Effects of thermochemical pretreatment on the anaerobic digestion of waste activated sludge. J. Water Sci.Technol. 35, 209–215 (1997)
Chen, Y.G., Jiang, S., Zhou, H.Y.Q., Gu, G.W.: Hydrolysis and acidification of waste activated sludge at different pHs. J. Water Res. 41, 683–689 (2007)
Church, F.C., Swaisgood, H.E., Catignani, G.L.: J. Appl. Biochem. 6, 205 (1984)
Aniello, A.D., Petrucelli, L., Gardner, C., Fisher, G.: Anal. Biochem. 213, 290 (1993)
Wang, Z.J., Wang, W., Zhang, X.H., Zhang, G.M.: Digestion of thermally hydrolyzed sewage sludge by anaerobic sequencing batch reactor. J. Hazard. Mater. 162, 799–803 (2009)
State Environment Protection Administration, China Environmental quality standards for surface water, GB 5086.2–1997. China Environmental Science Press, Beijing (1997)
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Su, R., Zhang, W., Wang, E., Zhu, M., Shi, P., Li, D. (2011). Optimized Process for Efficiently Extraction Protein from Excess Activated Sludge by Alcalase Hydrolysis. In: Lin, S., Huang, X. (eds) Advances in Computer Science, Environment, Ecoinformatics, and Education. CSEE 2011. Communications in Computer and Information Science, vol 215. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-23324-1_16
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DOI: https://doi.org/10.1007/978-3-642-23324-1_16
Publisher Name: Springer, Berlin, Heidelberg
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