Abstract
This paper proposes a method for automating the detection of lanes in Thin-Layer Chromatography images. Our approach includes a preprocessing step to detect the image region of interest, followed by background estimation and removal. This image is then projected onto the horizontal direction to integrate the information into a one-dimensional profile. A smoothing filter is applied to this profile and the outcome is the input of the lane detection process, which is performed in three phases. The first one aims at obtaining an initial set of candidate lanes that are further validated or removed in the second phase. The last phase is a refinement step that allows the inclusion of lanes that are not clearly distinguishable in the profile and that were not included in the initial set. The method was evaluated in 66 chromatography images and achieved values of recall, precision and F β -measure of 97.0%, 99.4% and 98.2%, respectively.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Fried, B., Sherma, J.: Thin-Layer Chromatography. Marcel Dekker, New York (1999)
Rodrigues, L.G., Ferraz, M.J., Rodrigues, D., Pais-Vieira, M., Lima, D., Brady, R.O., Sá-Miranda, M.C.: Neurophysiological behavioral and morphological abnormalities in the Fabry knockout mice. Neurobiology of Disease 33(1), 48–56 (2009)
Elstein, D., Altarescu, G., Beck, M.: Fabry Disease. Springer, Heidelberg (2010)
Houck, M.M., Siegel, J.A.: Fundamentals of Forensic Science. Elsevier, Oxford (2010)
Machado, A.M.C., Campos, M.F.M., Siqueira, A.M., de Carvalho, O.S.F.: An iterative algorithm for segmenting lanes in gel electrophoresis images. In: Proc. X Brazilian Symposium on Computer Graphics and Image Processing, pp. 140–146 (October 1997)
Elder, J.K., Southern, E.M.: Computer-aided analysis of one dimensional restriction fragment gels. In: Bishop, M.J., Rawlings, C.J. (eds.) Nucleic Acid and Protein Sequence Analysis - A Practical Aproach, pp. 165–172. IRL Press, Oxford (1987)
Bajla, I., Holländer, I., Fluch, S., Burg, K., Kollár, M.: An alternative method for electrophoretic gel image analysis in the GelMaster software. Computer Methods and Programs in Biomedicine 77, 209–231 (2005)
Lin, C., Ching, Y., Yang, Y.: An Automatic Method to Compare the Lanes in Gel Electrophoresis (GE) Images. IEEE Transaction on Information Technology in Biomedicine 11(2), 179–189 (2007)
Akbari, A., Fritz, A., Jackobsen, K.S.: Automatic lane detection and separation in one dimensional gel images using continuous wavelet transform. The Royal Society of Chemistry, Analytical Methods 2, 1360–1371 (2010)
Sousa, A.V., Aguiar, R.L., Mendonça, A.M., Campilho, A.C.: Automatic Lane and Band Detection in Images of Thin Layer Chromatography. In: Campilho, A.C., Kamel, M.S. (eds.) ICIAR 2004. LNCS, vol. 3212, pp. 158–165. Springer, Heidelberg (2004)
Sotaquira, M.: On the use of distance maps in the analysis of 1D DNA gel images. In: ICDIP 2009: Proceedings of the International Conference on Digital Image Processing, pp. 172–176. IEEE Computer Society (2009)
Barrantes, P., Alvarado, P.: Lane Detection on Gel Electrophoresis Images using Active Shape Models. In: Proc. of the Conference on Technologies for Sustainable Development, TSD 2011, pp. 43–46 (February 2011)
Mendonça, A.M., Sousa, A.V., Sá-Miranda, M.C., Campilho, A.C.: Automatic segmentation of chromatographic images for region of interest delineation. In: Proc. SPIE, vol. 7962, pp. 79623B1-79623B7 (2011)
Chau, F., Liang, Y., Gao, J., Shao, X.: Chemometrics – From Basics to Wavelet Transform. John Wiley & Sons, New Jersey (2004)
Press, W.H., Flannery, B.P., Teukolsky, S.A., Vetterling, W.T.: Numerical Recipes in C: The Art of Scientific Computing. Syndicate of the University of Cambridge (1992)
Soille, P., Jones, C.D., Smith, A.B., Roberts, E.F.: Morphological Image Analysis – Principles and Applications. Springer (2004)
Visa, S., Ralescu, A.: Learning Imbalanced and Overlapping Classes using Fuzzy Sets. In: Workshop on Learning from Imbalanced Datasets II, ICML (2003)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Moreira, B.M., Sousa, A.V., Mendonça, A.M., Campilho, A. (2012). Automatic Lane Detection in Chromatography Images. In: Campilho, A., Kamel, M. (eds) Image Analysis and Recognition. ICIAR 2012. Lecture Notes in Computer Science, vol 7325. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-31298-4_22
Download citation
DOI: https://doi.org/10.1007/978-3-642-31298-4_22
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-31297-7
Online ISBN: 978-3-642-31298-4
eBook Packages: Computer ScienceComputer Science (R0)