Abstract
This paper presents the results of a primary study that aims to produce miniaturized biosensing devices for nitrite analysis in clinical samples. Following our previous works regarding the development of amperometric nitrite biosensors using the nitrite reducing enzyme (ccNiR) from Desulfovibrio desulfuricans ATCC 27774, here we aimed at reducing the size of the experimental set-up according to the specific needs of biomedical applications. For this, thick-film strip electrodes made of carbon conductive inks deposited on plastic supports were modified with the ccNiR enzyme, previously mixed with the conductive graphite ink. Firstly, though, the electrode preparation was optimized (enzyme amount, organic solvent and curing temperature). Then, the biocompatibility of ccNiR with these harsh treatments and the analytical performance of the modified electrodes were evaluated by cyclic voltammetry. Finally, the carbon paste screen-printed electrodes were coated with the ccNiR/carbon ink composite, displaying a good sensitivity (5.3x10− 7 A.uM− 1.cm− 2) within the linear range of 0.001 - 1.5 mM.
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Correia, C. et al. (2013). Nitrite Biosensing Using Cytochrome C Nitrite Reductase: Towards a Disposable Strip Electrode. In: Gabriel, J., et al. Biomedical Engineering Systems and Technologies. BIOSTEC 2012. Communications in Computer and Information Science, vol 357. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-38256-7_3
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DOI: https://doi.org/10.1007/978-3-642-38256-7_3
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