Abstract
To analyze the intracellular movements of subviral particles (nucleocapsids, NCs) of the Marburg virus, the viral protein VP30 has been labeled fluorescently. This makes the NCs observable by fluorescence microscopy under biosafety level 4 conditions. An algorithm has been developed, aiming to allow the automated detection and tracking of the NCs . The specific feature of this approach is the inclusion of expertise about the NCs’ appearance and movement characteristics, what gives more reliable results than a simple nearest neighbor linking of the detected NCs.
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Kienzle, C., Schudt, G., Becker, S., Schanze, T. (2014). Multiple Subviral Particle in Fluorecsence Microscopy Sequences. In: Deserno, T., Handels, H., Meinzer, HP., Tolxdorff, T. (eds) Bildverarbeitung für die Medizin 2014. Informatik aktuell. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54111-7_61
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DOI: https://doi.org/10.1007/978-3-642-54111-7_61
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