Abstract
The prediction of subcellular localisation of proteins is one of the main goals of proteome sequencing, and researchers have achieved high classification accuracy with the help of computer technology, but most of the current classification models are not applicable to the classification of plant vacuole proteins, and it is tedious and time-consuming to classify plant vacuole proteins using subcellular localisation methods. In this paper, we focus on the classification of plant vacuole proteins based on an ensemble stacking model. New feature inputs are generated by fusing statistical and physicochemical features of proteins. The data is accurately classified by using an ensemble stacking model based on a number of machine learning algorithms. The results show that the model achieves a classification accuracy of 73%, which is a significant advance compared to other models and is of high significance for studying the classification of plant vacuole proteins.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Boden, M., Hawkins, J.: Prediction of subcellular localization using sequence-biased recurrent networks. Bioinformatics 21, 2279–2286 (2005)
Chou, K.C., Shen, H.B.: Plant-mPLoc: a top-down strategy to augment the power for predicting plant protein subcellular localization. PLoS ONE 5, e11335 (2010)
Nakashima, H., Nishikawa, K.: The amino acid composition is different between the cytoplasmic and extracellular sides in membrane proteins. FEBS Lett. 303 (1992)
Guo, J., Lin, Y., Sun, Z.: A novel method for protein subcellular localization: combining residue-couple model and SVM. In: Asia-Pacific Bioinformatics Conference, Singapore, pp. 117–129 (2005)
Chou, K.C.: Prediction of protein cellular attributes using pseudo-amino acid composition. Proteins: Struct. Funct. Bioinf. 43(3), 246–255 (2001)
Chou, K.C., Shen, H.B.: Predicting protein subcellular location by fusing multiple classifiers. J Cell Biochem. 99(2), 517–527 (2006)
Breiman, L.: Random forest. Mach. Learn. 45(1), 5–32 (2001)
Wang, Y.-C., Wang, Y., Yang, Z.-X., et al.: Support vector machine prediction of enzyme function with conjoint triad feature and hierarchical context. BMC Syst. Biol. 5(S1), S6 (2011)
Ho, T.K.: The random subspace method for constructing decision forests. IEEE Trans. Pattern Anal. Mach. Intell. 20(8), 832–844 (1998)
Pedregosa, F., et al.: Scikit-learn: machine learning in Python. J. Mach. Learn. Res. 12, 2825–2830 (2011)
Wei, L., Xing, P., Zeng, J., Chen, J., Su, R., Guo, F.: Improved prediction of protein–protein interactions using novel negative samples, features, and an ensemble classifier. Artif. Intell. Med. 83, 67–74 (2017)
Wei, L., Xing, P., Su, R., Shi, G., Ma, Z.S., Zou, Q.: CPPred-RF: a sequence-based predictor for identifying cell-penetrating peptides and their uptake efficiency. J. Proteome Res. 16(5), 2044–2053 (2017)
Zhang, C., Hicks, G., Raikhel, N.: Molecular composition of plant vacuoles: important but less understood regulations and roles of tonoplast lipids. Plants 4, 320–333 (2015)
Zhang, C., Hicks, G.R., Raikhel, N.V.: Plant vacuole morphology and vacuolar trafficking. Front. Plant Sci. 5, 476 (2014)
Zhang, L., Zhao, X., Kong, L.: Predict protein structural class for low-similarity sequences by evolutionary difference information into the general form of Chou’s pseudo amino acid composition. J. Theor. Biol. 355, 105–110 (2014)
Acknowledgments
This work was supported by the National Natural Science Foundation of China (Grant No. 61902337), Xuzhou Science and Technology Plan Project (KC21047), Jiangsu Provincial Natural Science Foundation (No. SBK2019040953), Natural Science Fund for Colleges and Universities in Jiangsu Province (No. 19KJB520016) and Young Talents of Science and Technology in Jiangsu and ghfund202302026465.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
About this paper
Cite this paper
Ju, X., Xiao, K., He, L., Wang, Q., Wang, Z., Bao, W. (2023). Plant Vacuole Protein Classification with Ensemble Stacking Model. In: Huang, DS., Premaratne, P., Jin, B., Qu, B., Jo, KH., Hussain, A. (eds) Advanced Intelligent Computing Technology and Applications. ICIC 2023. Lecture Notes in Computer Science, vol 14088. Springer, Singapore. https://doi.org/10.1007/978-981-99-4749-2_53
Download citation
DOI: https://doi.org/10.1007/978-981-99-4749-2_53
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-99-4748-5
Online ISBN: 978-981-99-4749-2
eBook Packages: Computer ScienceComputer Science (R0)