Abstract
Recently, the technique of fish bioassay has attracted attention as a method for constant monitoring of aquatic contamination. The respiratory rhythms of fish are considered to be an efficient indicator for the monitoring of water quality, since they are sensitive to chemicals and can be measured indirectly from the bioelectric signals generated by their breathing. However, no method has yet been established to measure signals in small free-swimming fish. In this article, we propose a system to measure bioelectric signals in small fish and monitor the frequency component in real time. To cover the large measurement range required in a free-swimming environment, the signals are measured using multiple electrodes. Further, the system focuses on the frequency component of the signal to assess the condition of the fish using frequency analysis and a band-pass filter. Experiments were conducted with the purpose of enabling remote sensing and environment estimation. First, it was verified that the measured signals were synchronized with the breathing of the fish. Then, a remote sensing experiment was performed using medaka (Oryzias latipes) that were allowed to swim freely in a measurement aquarium. The results confirmed that bioelectric signals which were synchronized with breathing could be measured in unconstrained and noninvasive conditions.
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Ministry of Health, Labour and Welfare (2007) Enforcement regulation of water supply law (in Japanese)
Ministry of Health, Labour and Welfare (2001) Anti-terrorism policy (in Japanese) http://www.mhlw.go.jp/houdou/0110/h1005-1.html, Accessed 16 July 2007
Thomas M, Florion A (1996) A new warning biomonitor using a weak electric fish, Apteronotus albifrons (Gymnotiformes), and the effect of temperature on the bioelectric responses. Environ Monitoring Assess 51:605–620
Shedd TR, van der Schalie WH (2001) Long-term operation of an automated fish biomonitoring system for continuous effluent acute toxicity surveillance. Burrows Bull Environ Contaminat Toxicol 66:392–399
van der Schalie WH, Shedd TR (2004) Response characteristics of an aquatic biomonitor used for rapid toxicity detection. J Appl Toxicol 24:387–394
Taue S, Hashimoto R (2006) Unirelief water quality monitoring system (in Japanese). J Resourc Environ 42(2):73–77
Organisation for Economic Co-operation and Development (OECD) (1992) OECD guidelines for the testing of chemicals No. 203. Fish: acute toxicity test
Peters RC, Loos WJG, Gerritsen A (1974) Distribution of electroreceptors, bioelectric field patterns, and skin resistance in the catfish, Ictalurus nebulosus LeS. J Comp Physiol A 92:11–22
Wood CM, Part P (1997) Cultured branchial epithelia from freshwater fish gills. J Exp Biol 200:1047–1059
Peters RC, Buwalda RJA (1972) Frequency response of the electroreceptors (“small pit organs”) of the catfish Ictalurus nebulosus LeS. J Comp Physiol A 79:29–38
Umezawa S, Watanabe H (1973) On the respiration of the killifish Oryzias latipes. J Exp Biol 58:305–325
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This work was presented in part at the 14th International Symposium on Artificial Life and Robotics, Oita, Japan, February 5–7, 2009
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Terawaki, M., Hirano, A., Soh, Z. et al. Unconstrained and noninvasive measurement of bioelectric signals from small fish. Artif Life Robotics 14, 342–347 (2009). https://doi.org/10.1007/s10015-009-0678-x
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DOI: https://doi.org/10.1007/s10015-009-0678-x