Skip to main content
Springer Nature Link
Log in

Improved Techniques for a Murine Model of Myocardial Ischemia

  • Original Paper
  • Published:
Journal of Medical Systems Aims and scope Submit manuscript

Abstract

Objective

Our study investigated a rapid and reliable method of surgical occlusion of coronary vessels in mice. The study improves the chance of success in making a myocardial ischemia model in mice, and provides a novel method to a novice and laboratory with limited conditions.

Methods and Results

Sixty mice were evenly divided into two groups, a modified group (oral intubation and self-made rib retractor) and a conventional group (tracheotomy and rib cutting). During the perioperative period, the success rate of model establishment and the survival rate of the mice in the modified group were significantly higher than those in the conventional group (P  < 0.01). Also, the status of the mice in the modified group after operation was better than that of the conventional group. Moreover, operation times in the modified group were significantly shorter than those of the conventional group (P  < 0.01). The infarct size, as assessed using triphenyltetrazolium chloride staining, was similar between the two groups (P  > 0.05).

Conclusion

This novel method is simple and efficient and can be conducted independently, enhancing the success rate of myocardial ischemic model establishment in mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Lardenoye, JH, de Vries, MR, Lowik, CW, Xu, Q, Dhore, CR, Cleutjens, JP, van Hinsbergh, VW, van Bockel, JH, and Quax, PH, Accelerated atherosclerosis and calcification in vein grafts: a study in APOE*3 Leiden transgenic mice. Circ Res. 91:577–584, 2002. doi:10.1161/01.RES.0000036901.58329.D7.

    Article  Google Scholar 

  2. Kuhlencordt, PJ, Gyurko, R, Han, F, Scherrer-Crosbie, M, Aretz, TH, Hajjar, R, Picard, M, and Huang, PL, Accelerated atherosclerosis, aortic aneurysm formation, and ischemic heart disease in apolipoprotein E/endothelial nitric oxide synthase double-knockout mice. Circulation. 104:448–454, 2001. doi:10.1161/hc2901.091399.

    Article  Google Scholar 

  3. Mach, F, Sukhova, GK, Michetti, M, Libby, P, and Michetti, P, Influence of Helicobacter pylori infection during atherogenesis in vivo in mice. Circ Res. 90:E1–E4, 2002. doi:10.1161/hh0102.102270.

    Article  Google Scholar 

  4. Ma, N, Stamm, C, Kaminski, A, Li, W, Kleine, HD, Muller-Hilke, B, Zhang, L, Ladilov, Y, Egger, D, and Steinhoff, G, Human cord blood cells induce angiogenesis following myocardial infarction in NOD/scid-mice. Cardiovasc Res. 66:45–54, 2005. doi:10.1016/j.cardiores.2004.12.013.

    Article  Google Scholar 

  5. Salto-Tellez, M, Yung Lim, S, El-Oakley, RM, Tang, TP, ALmsherqi, ZA, and Lim, SK, Myocardial infarction in the C57BL/6J mouse: a quantifiable and highly reproducible experimental model. Cardiovasc Pathol. 13:91–97, 2004. doi:10.1016/S1054-8807(03)00129-7.

    Article  Google Scholar 

  6. Wang, J, Bo, H, Meng, X, Wu, Y, Bao, Y, and Li, Y, A simple and fast experimental model of myocardial infarction in the mouse. Tex Heart Inst J. 33:290–293, 2006.

    Google Scholar 

  7. Michael, LH, Entman, ML, Hartley, CJ, Youker, KA, Zhu, J, Hall, SR, Hawkins, HK, Berens, K, and Ballantyne, CM, Myocardial ischemia and reperfusion: a murine model. Am J Physiol. 269:H2147–H2154, 1995.

    Google Scholar 

  8. Toyota, E, Kawaguchi, Y, Ogasawara, Y, Watanabe, N, Neishi, Y, Kawamoto, T, Okura, H, and Yoshida, K, Novel rat model of ischemic cardiomyopathy induced by repetitive myocardial ischemia/reperfusion injury while conscious. Circ J. 71:788–795, 2007. doi:10.1253/circj.71.788.

    Article  Google Scholar 

  9. Jong, WM, Reitsma, PH, ten Cate, H, and de Winter, RJ, Modified two-step model for studying the inflammatory response during myocardial ischemia and reperfusion in mice. Comp Med. 53:522–526, 2003.

    Google Scholar 

  10. Akhter, SA, Luttrell, LM, Rockman, HA, Iaccarino, G, Lefkowitz, RJ, and Koch, WJ, Targeting the receptor-Gq interface to inhibit in vivo pressure overload myocardial hypertrophy. Science. 280:574–577, 1998. doi:10.1126/science.280.5363.574.

    Article  Google Scholar 

  11. Zhao, X, Wu, N, Zhou, J, Yang, Y, Fang, Y, Cheng, W, Ma, R, Tian, Y, and Huang, L, A technique for retrograde intubation in mice. Lab Anim (NY). 35:39–42, 20206. doi:10.1038/laban0306-39.

    Article  Google Scholar 

  12. Brown, RH, Walters, DM, Greenberg, RS, and Mitzner, W, A method of endotracheal intubation and pulmonary functional assessment for repeated studies in mice. J Appl Physiol. 87:2362–2365, 1999.

    Google Scholar 

Download references

Acknowledgments

This study was supported by the grants from National Natural Science Foundation of China (NO.30600580).

Author information

Authors and Affiliations

  1. Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, China

    Yuan Yuan, Fei Li, Li-Ping Wei, Rong-Qing Zhang & Hai-Chang Wang

Authors
  1. Yuan Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Li-Ping Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rong-Qing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hai-Chang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Fei Li or Hai-Chang Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yuan, Y., Li, F., Wei, LP. et al. Improved Techniques for a Murine Model of Myocardial Ischemia. J Med Syst 34, 413–417 (2010). https://doi.org/10.1007/s10916-009-9254-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10916-009-9254-0

Keywords