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Extraction of target region in lung immunohistochemical image based on artificial neural network

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Abstract

Immunohistochemistry is widely used in clinical pathology analysis and diagnosis, the target regions segmentation is the key procedure and always provides support for many qualitative and quantitative analyses on digitized immunohistochemical image. In lung tissue immunohistochemistry applications, the target region needs to be extracted out of the whole image firstly. Most existing methods based on color cannot fulfill the extraction of antibody region. Thus, there is a need of effective extraction method. Methods: According to the features of target region in images to be processed, this paper presents a solution framework based on artificial neural network (ANN). Results: Six effective features of the candidate regions are analyzed and extracted as the inputs of the ANN; three-layers back propagation neural network with six inputs and one output is constructed, and ANN’s parameters are trained by the learning image set. By the trained ANN, target region core are obtained and then expanded to the whole target region through conditional expansion. Conclusion: Through testing the framework by testing image set and comparing with the main existing methods, it can be concluded that the proposed framework can remove non-target regions and extract the target regions well, while the contrast methods cannot remove all the non-target regions. Significance: The method presented in this paper has practical and potential significance to realize automated and quantitative tissue immunohistochemical image analysis.

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Acknowledgments

This paper partially aided by the Shandong Province Young Scientist Foundation (BS2012DX034, BS2013NJ023), China Postdoctoral Science Foundation (2012 M521361), Shandong Province Natural Science Foundation (ZR2012EEM021), Project of Shandong Province Higher Educational Science and Technology Program (J13LN17), Graduate Student Science and Technology Innovation Fund of SDUST(YC140212), and Project of South Africa/China Research Collaboration in Science and Technology (2012DFG71060).

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  1. Shandong University of Science and Technology, 579 Qianwangang Road, Economic & Technical Development Zone, Qingdao, 266590, China

    Di Fan, Lu Wei & Maoyong Cao

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  1. Di Fan
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  2. Lu Wei
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  3. Maoyong Cao
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Correspondence to Maoyong Cao.

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Fan, D., Wei, L. & Cao, M. Extraction of target region in lung immunohistochemical image based on artificial neural network. Multimed Tools Appl 75, 12227–12244 (2016). https://doi.org/10.1007/s11042-016-3459-2

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  • DOI: https://doi.org/10.1007/s11042-016-3459-2

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