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Recent technological advancements related to articular cartilage regeneration

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Abstract

Some treatments for full thickness defects of the articular cartilage, such as the transplantation of cultured chondrocytes have already been performed. However, in order to overcome osteoarthritis, we must further study the partial thickness defects of articular cartilage. It is much more difficult to repair a partial thickness defect because few repair cells can address such injured sites. We herein show that bioengineered and layered chondrocyte sheets using temperature-responsive culture dishes may be a potentially useful treatment for the repair of partial thickness defects. We also show that a chondrocyte-plate using a rotational culture system without the use of a scaffold may also be useful as a core cartilage of an articular cartilageous defect. We evaluated the properties of these sheets and plates using histological findings, scanning electrical microscopy, and photoacoustic measurement methods, which we developed to evaluate the biomechanical properties of tissue-engineered cartilage. In conclusion, the layered chondrocyte sheets and chondrocyte-plates were able to maintain the cartilageous phenotype, thus suggesting that they could be a new and potentially effective therapeutic product when attached to the sites of cartilage defects.

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Acknowledgments

This work was supported by the Takeda Science Foundation, High-Tech Research Center Project 2004 for Private Universities, and a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, the New Energy and Industrial Technology Development Organization, and Japan Foundation for Aging and Health.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan

    Masato Sato, Miya Ishihara, Nagatoshi Kaneshiro, Toshihiro Nagai, Genya Mitani, Toshiharu Kutsuna, Naoshi Ohta, Mami Kokubo & Joji Mochida

  2. Department of Medical Engineering, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan

    Miya Ishihara & Makoto Kikuchi

  3. Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8656, Japan

    Katsuko Furukawa

  4. Department of Mechanical Engineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8656, Japan

    Katsuko Furukawa

  5. Laboratory of Regenerative Medical Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo, 113-8656, Japan

    Takashi Ushida

  6. CellSeed Inc., 33-8 Wakamatsucho, Shinjuku, Tokyo, 162-0056, Japan

    Tetsutaro Kikuchi & Hideaki Sakai

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  1. Masato Sato
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  2. Miya Ishihara
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  3. Katsuko Furukawa
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  4. Nagatoshi Kaneshiro
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Correspondence to Masato Sato.

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Sato, M., Ishihara, M., Furukawa, K. et al. Recent technological advancements related to articular cartilage regeneration. Med Biol Eng Comput 46, 735–743 (2008). https://doi.org/10.1007/s11517-008-0360-9

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  • DOI: https://doi.org/10.1007/s11517-008-0360-9

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