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NIR spectroscopic determination of urine components in spot urine: preliminary investigation towards optical point-of-care test

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Abstract

Presently, there is no convenient method to measure 24-h urinary Na excretion, which is an important index of daily Na intake, and 24-h urine collection involves a complex process. However, the Na-to-creatinine ratio (NCR) in spot urine has the potential to evaluate 24-h Na excretion and is useful for point-of-care testing. Thus, this study aimed to realize a near-infrared spectroscopic system to assess NCR in spot urine: (1) We attempted to estimate Na concentration using fewer than 10 wavelengths; (2) we calculated NCR using creatinine concentrations from our previous report and verified the NCR predictability. A calibration model was created using multi-linear regression analysis using 10 selected wavelengths in the range of Fourier-transform infrared spectrometer. Spot urine samples were obtained from healthy adults, and glucose powder was added to them to simulate diabetic samples. NCR was calculated using only six wavelengths, and the results confirmed the high accuracy of the estimated Na concentration even though inorganic components do not absorb near-infrared light. Our method enables to optically estimate NCR in spot urine, and it will be useful for point-of-care testing.

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Acknowledgments

The authors would like to appreciate the urine sample donors and the graduates of the Division of Mechanical Science and Engineering, Kanazawa University, for their technical assistance.

Funding

This work was partly supported by Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (B) 20300195, 26282119, 18H03514, and (C) 17 K01407.

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Authors and Affiliations

  1. Graduate School of Natural Science & Technology, Kanazawa University, Kanazawa, 920-1192, Japan

    Ikuto Suzuki, Kimihiro Seino & Ken-ichi Yamakoshi

  2. Department of Information and Electronic Engineering, Faculty of Science and Engineering, Teikyo University, Utsunomiya, 320-8551, Japan

    Mitsuhiro Ogawa

  3. Department of Clinical Engineering, Faculty of Health Sciences, Komatsu University, Komatsu, 923-0961, Japan

    Masamichi Nogawa

  4. Faculty of Frontier Engineering, Institute of Science & Engineering, Kanazawa University, Kanazawa, 920-1192, Japan

    Hisashi Naito & Shinobu Tanaka

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  1. Ikuto Suzuki
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Correspondence to Ikuto Suzuki.

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Suzuki, I., Ogawa, M., Seino, K. et al. NIR spectroscopic determination of urine components in spot urine: preliminary investigation towards optical point-of-care test. Med Biol Eng Comput 58, 67–74 (2020). https://doi.org/10.1007/s11517-019-02063-1

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