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Analysis of non-physiological shear stress-induced red blood cell trauma across different clinical support conditions of the blood pump

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Abstract

Systematic research into device-induced red blood cell (RBC) damage beyond hemolysis, including correlations between hemolysis and RBC-derived extracellular vesicles, remains limited. This study investigated non-physiological shear stress-induced RBC damage and changes in related biochemical indicators under two blood pump clinical support conditions. Pressure heads of 100 and 350 mmHg, numerical simulation methods, and two in vitro loops were utilized to analyze the shear stress and changes in RBC morphology, hemolysis, biochemistry, metabolism, and oxidative stress. The blood pump created higher shear stress in the 350-mmHg condition than in the 100-mmHg condition. With prolonged blood pump operation, plasma-free hemoglobin and cholesterol increased, whereas plasma glucose and nitric oxide decreased in both loops. Notably, plasma iron and triglyceride concentrations increased only in the 350-mmHg condition. The RBC count and morphology, plasma lactic dehydrogenase, and oxidative stress across loops did not differ significantly. Plasma extracellular vesicles, including RBC-derived microparticles, increased significantly at 600 min in both loops. Hemolysis correlated with plasma triglyceride, cholesterol, glucose, and nitric oxide levels. Shear stress, but not oxidative stress, was the main cause of RBC damage. Hemolysis alone inadequately reflects overall blood pump-induced RBC damage, suggesting the need for additional biomarkers for comprehensive assessments.

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Data availability

All data generated or analyzed during this study are included in this published article and its supplementary information files. The original datasets used and analyzed during the current study can be obtained from the corresponding author on reasonable request.

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Funding

This work was supported by the National Key R&D Program of China (Grant no. 2020YFC0862904, 2020YFC0862902, and 2020YFC0862900), National Natural Science Foundation of China (Grant no.12372300, 32071311) and Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Room 223, Building 5, No.37 Xueyuan Road, Haidian District, Beijing, 100083, China

    Xinyu Liu, Yuan Li, Jinze Jia, Hongyu Wang, Yifeng Xi, Anqiang Sun, Lizhen Wang, Xiaoyan Deng, Zengsheng Chen & Yubo Fan

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  1. Xinyu Liu
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  2. Yuan Li
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Contributions

Xinyu Liu: Experimentation, Data collection, Data analysis/interpretation, Statistical analysis, Drafting of article, Critical revision of article; Yuan Li: CFD analysis, Critical revision of article, Approval of article; Jinze Jia: Experimentation, Critical revision of article, Approval of article; Hongyu Wang: Experimentation, Critical revision of article, Approval of article; Yifeng Xi: Experimentation, Critical revision of article, Approval of article; Anqaing SUN: Critical revision of article, Approval of article, Funding acquisition; Lizheng WANG: Critical revision of article, Approval of article, Funding acquisition; Xiaoyan DENG: Critical revision of article, Approval of article, Funding acquisition; Zengsheng CHEN: Critical revision of article, Approval of article, Funding acquisition; and Yubo FAN: Critical revision of article, Approval of article, Funding acquisition.

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Correspondence to Zengsheng Chen or Yubo Fan.

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Liu, X., Li, Y., Jia, J. et al. Analysis of non-physiological shear stress-induced red blood cell trauma across different clinical support conditions of the blood pump. Med Biol Eng Comput 62, 3209–3223 (2024). https://doi.org/10.1007/s11517-024-03121-z

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