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All-optical quantitative framework for bioluminescence tomography with non-contact measurement

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Abstract

In this contribution, we present an all-optical quantitative framework for bioluminescence tomography with non-contact measurement. The framework is comprised of four indispensable steps: extraction of the geometrical structures of the subject, light flux reconstruction on arbitrary surface, calibration and quantification of the surface light flux and internal bioluminescence reconstruction. In particular, the geometrical structures are retrieved using a completely optical method and captured under identical viewing conditions with the bioluminescent images. As a result, the proposed framework avoids the utilization of computed tomography or magnetic resonance imaging to provide the geometrical structures. On the basis of experimental measurements, we evaluate the performance of the proposed all-optical quantitative framework using a mouse shaped phantom. Preliminary result reveals the potential and feasibility of the proposed framework for bioluminescence tomography.

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Authors and Affiliations

  1. Life Sciences Research Center, School of Life Sciences and Technology, Xidian University, Xi’an, 710071, PRC

    Xue-Li Chen, Heng Zhao, Xiao-Chao Qu, Duo-Fang Chen & Ji-Min Liang

  2. School of Technical Physics, Xidian University, Xi’an, 710071, PRC

    Xiao-Rui Wang

Authors
  1. Xue-Li Chen
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  2. Heng Zhao
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  3. Xiao-Chao Qu
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  4. Duo-Fang Chen
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  5. Xiao-Rui Wang
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  6. Ji-Min Liang
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Corresponding author

Correspondence to Ji-Min Liang.

Additional information

This work was supported by National Basic Research Program of China (973 Program) (No. 2011CB707702), National Natural Science Foundation of China (No. 81090272, No. 81000632, and No. 30900334), Shaanxi Provincial Natural Science Foundation Research Project (No. 2009JQ8018), and Fundamental Research Funds for the Central Universities.

Xue-Li Chen graduated from Xidian University, PRC in 2007. He received the B. Eng. degree in biomedical engineering from Xidian University, Xi’an, PRC in 2007. In 2007, he started the successive postgraduate and doctoral programs of study in pattern recognition and intelligent system, at the School of Electronic Engineering, Xidian University.

His research interests include optical imaging, photon transport in biological tissues, and in free-space.

Heng Zhao graduated from Xi’an Jiaotong University, PRC in 1996. He received the B. Sc. degree in automatic control from Xi’an Jiaotong University in 1996, and the Ph.D. degree in circuit and system from Xidian University in 2005. Currently, he is an associate professor in the School of Life Sciences and Technology at Xidian University.

His research interests include biomedical image processing and biometric recognition.

Xiao-Chao Qu graduated from Xidian University, PRC in 2003. She received the B. Eng. degree in biomedical engineering in 2003 from Xidian University, Ph.D. degree in Biomedical engineering in 2008 from Xi’an Jiaotong University. Currently, she is an associate professor in the School of Life Sciences and Technology at Xidian University.

Her research interests include multimodality molecular imaging and biomedical photonics.

Duo-Fang Chen graduated from Xidian University, PRC in 2004. She received the B. Sc. and Ph.D. in applied physics and signal and information processing from Xidian University, in 2004 and 2009, respectively. Since 2009, she has been a lecturer in Life Sciences Research Center, School of Life Sciences and Technology, Xidian University.

Her research interests include signal processing and molecular imaging.

Xiao-Rui Wang graduated from Sichuan University, PRC in 1998. He received the B. Eng. degree in optoelectronic technology, from Sichuan University, Chengdu, PRC in 1998. In 2005, he received the Ph.D degree in optical engineering from Xidian University, Xi’an China. Currently, he is a professor in the School of Technical Physics at Xidian University. In 2007, he was a visiting scholar at 3D Visualization and Imaging System Laboratory, University of Arizona. He is a member of Optical Society of America.

His research interests include three-dimensional optical imaging and visualization, optoelectronic imaging and detection technology, and development of optical imaging system.

Ji-Min Liang received the B.Eng. degree in automatic control in 1992, M.Eng. degree in signal and information processing in 1995, and Ph.D. in circuits and systems in 1999, all from Xidian University. He became an associate professor and then professor in 2000 and 2005, respectively. In 2002, he was a research associate professor at the Electrical and Computer Engineering Department, University of Tennessee, Knoxville, USA. He joined the School of Life Sciences and Technology in 2009. He is a member of IEEE.

His research interests include multimodality molecular imaging, biomedical image processing, biometric recognition, and biometric encryption.

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Chen, XL., Zhao, H., Qu, XC. et al. All-optical quantitative framework for bioluminescence tomography with non-contact measurement. Int. J. Autom. Comput. 9, 72–80 (2012). https://doi.org/10.1007/s11633-012-0618-4

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  • DOI: https://doi.org/10.1007/s11633-012-0618-4

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