Abstract
Type II DNA topoisomerases can catalyze the transport of one DNA segment through a transient break in another DNA segment by a complex mechanism of ATP hydrolysis. According to the hydrolysis process of two ATPs, a multi-state model is proposed to investigate the work cycle of DNA topoisomerase II. The rate of the opening and closing of the DNA topoisomerase gate is evaluated by determining the release rate of inorganic phosphates. The calculated results show that, under the condition of the high concentration of ATP, the work cycle of DNA topoisomerase II is about 0.84 s which is in agreement with the experimental data.
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Acknowledgments
Supported by the National Natural Science Foundation of China (Grant No. 11047180 and 20973034), the Fundamental Research Funds for the Central Universities (ZYGX2009J081) and Scientific Research Startup Foundation of UESTC.
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Ding, H., Lin, H. & Feng, J. The rate of opening and closing of the DNA gate for topoisomerase II. Theory Biosci. 132, 61–64 (2013). https://doi.org/10.1007/s12064-012-0163-2
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DOI: https://doi.org/10.1007/s12064-012-0163-2