Abstract
Digital PCR generally includes two parts. That is, the PCR amplification and fluorescence signal analysis. In the PCR amplification stage. Using digital PCR to dilute samples onto a single molecule level, the average to dozens to tens of thousands of units in chemical reaction, make each reaction unit of target molecules is 0 or 1, makes the target molecules positive signal detection. One of them will use the upper software control laboratory PCR instrument operation, instrument of the data acquisition, as well as to the scientific analysis and processing the data collected, is advantageous for the quantitative analysis of the gene and detection. The software to control one PCR instrument operation and work. In addition this instrument will collect data uploaded to PC through USB interface, through analysis and deal with the PC software. Finally experimental conclusions.
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Li, X., Wu, S., Wang, M. et al. The software system design model based on digital PCR fluorescence detector. Cluster Comput 22 (Suppl 4), 8623–8627 (2019). https://doi.org/10.1007/s10586-018-1931-7
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DOI: https://doi.org/10.1007/s10586-018-1931-7