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Methods to Quantify DNA Transfer in Enterococcus

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Horizontal Gene Transfer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2075))

Abstract

DNA uptake in Enterococcus normally occurs by conjugation, a natural process that is replicated in biomedical research to assess the transferability of different mobile genetic elements and chromosomal regions as well as to study the host range of plasmids and other conjugative elements. More efficient artificial methods to transform cells with foreign DNA as chemotransformation and electroporation are widely used in molecular genetics. Here, we described conjugation protocols to quantify DNA transfer among Enterococcus and revise current perspectives and lab strains. Protocols of electrotransformation have been previously described in this series.

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Author information

Authors and Affiliations

  1. Laboratory of Microbiology, Biological Sciences Department, Faculty of Pharmacy, UCIBIO/REQUIMTE, University of Porto, Porto, Portugal

    Carla Novais, Ana R. Freitas & Luísa Peixe

  2. Department of Microbiology, Ramón y Cajal Health Research Institute (IRYCIS), Unidad de Resistencia a Antibióticos y Virulencia Bacteriana (RYC-CSIC), CIBER en Epidemiología y Salud Pública (CIBER-ESP), Ramón y Cajal University Hospital, Madrid, Spain

    Ricardo León-Sampedro

  3. Department of Microbiology, Ramón y Cajal Research Institute (IRYCIS), Madrid, Spain

    Teresa M. Coque

  4. CIBER in Epidemiology and Public Health (CIBERESP), Madrid, Spain

    Teresa M. Coque

Authors
  1. Carla Novais
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  2. Ana R. Freitas
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  3. Ricardo León-Sampedro
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  4. Luísa Peixe
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  5. Teresa M. Coque
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Corresponding authors

Correspondence to Carla Novais or Teresa M. Coque .

Editor information

Editors and Affiliations

  1. Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria-CSIC, Santander, Cantabria, Spain

    Fernando de la Cruz

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Novais, C., Freitas, A.R., León-Sampedro, R., Peixe, L., Coque, T.M. (2020). Methods to Quantify DNA Transfer in Enterococcus. In: de la Cruz, F. (eds) Horizontal Gene Transfer. Methods in Molecular Biology, vol 2075. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9877-7_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9877-7_8

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9876-0

  • Online ISBN: 978-1-4939-9877-7

  • eBook Packages: Springer Protocols

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