Fiber based in-vivo imaging of epithelial FAD fluorescence: experiments and simulations

Bala Nivetha Kanakaraj; Sujatha Narayanan Unni

doi:10.1117/12.2080561

17 March 2015 Fiber based in-vivo imaging of epithelial FAD fluorescence: experiments and simulations

Bala Nivetha Kanakaraj, Sujatha Narayanan Unni

Proceedings Volume 9417, Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging; 94171X (2015) https://doi.org/10.1117/12.2080561
Event: SPIE Medical Imaging, 2015, Orlando, Florida, United States

Abstract

Fluorescence from endogenous fluorophores has been emerging as a promising biomarker for tissue discrimination resulting a noninvasive screening methodology to understand the biochemical and morphological variations in tissues associated with cancer development. We have developed a scan based fiber optic probe system to image increased flavin adenine dinucleotide (FAD) fluorescence from epithelial tissues under conditions mimicking dysplasia surrounded by normal tissues. Experiments were conducted on optical phantoms mimicking epithelial tissues excited by 450nm LED source. The spectral emission from the sample is collected via optical fibers and the imaging is performed by scanning the sample using a translation stage at desired resolution. Monte Carlo simulations were also performed by devising an optical model corresponding to epithelial tissue and the results were correlated with experimental fluorescence measurements. This whole field imaging approach could be useful for in vivo assessment of tissue pathologies based on auto fluorescence and can give a better quantitative approach for estimation of tissue properties by correlating the experimental and simulated data.

Citation Download Citation

Bala Nivetha Kanakaraj and Sujatha Narayanan Unni "Fiber based in-vivo imaging of epithelial FAD fluorescence: experiments and simulations", Proc. SPIE 9417, Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging, 94171X (17 March 2015); https://doi.org/10.1117/12.2080561

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