Automated epidermis segmentation in histopathological images of human skin stained with hematoxylin and eosin

Paweł Kłeczek; Grzegorz Dyduch M.D.; Joanna Jaworek-Korjakowska; Ryszard Tadeusiewicz

doi:10.1117/12.2249018

1 March 2017 Automated epidermis segmentation in histopathological images of human skin stained with hematoxylin and eosin

Paweł Kłeczek, Grzegorz Dyduch M.D., Joanna Jaworek-Korjakowska, Ryszard Tadeusiewicz

Author Affiliations +

Proceedings Volume 10140, Medical Imaging 2017: Digital Pathology; 101400M (2017) https://doi.org/10.1117/12.2249018
Event: SPIE Medical Imaging, 2017, Orlando, Florida, United States

Abstract

Background: Epidermis area is an important observation area for the diagnosis of inflammatory skin diseases and skin cancers. Therefore, in order to develop a computer-aided diagnosis system, segmentation of the epidermis area is usually an essential, initial step. This study presents an automated and robust method for epidermis segmentation in whole slide histopathological images of human skin, stained with hematoxylin and eosin. Methods: The proposed method performs epidermis segmentation based on the information about shape and distribution of transparent regions in a slide image and information about distribution and concentration of hematoxylin and eosin stains. It utilizes domain-specific knowledge of morphometric and biochemical properties of skin tissue elements to segment the relevant histopathological structures in human skin. Results: Experimental results on 88 skin histopathological images from three different sources show that the proposed method segments the epidermis with a mean sensitivity of 87 %, a mean specificity of 95% and a mean precision of 57%. It is robust to inter- and intra-image variations in both staining and illumination, and makes no assumptions about the type of skin disorder. The proposed method provides a superior performance compared to the existing techniques.

Citation Download Citation

Paweł Kłeczek, Grzegorz Dyduch M.D., Joanna Jaworek-Korjakowska, and Ryszard Tadeusiewicz "Automated epidermis segmentation in histopathological images of human skin stained with hematoxylin and eosin", Proc. SPIE 10140, Medical Imaging 2017: Digital Pathology, 101400M (1 March 2017); https://doi.org/10.1117/12.2249018

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available