Non-rigid registration of multiphoton microscopy images using B-splines

Kevin S. Lorenz; Paul Salama; Kenneth W. Dunn; Edward J. Delp

doi:10.1117/12.878304

11 March 2011 Non-rigid registration of multiphoton microscopy images using B-splines

Kevin S. Lorenz, Paul Salama, Kenneth W. Dunn, Edward J. Delp

Proceedings Volume 7962, Medical Imaging 2011: Image Processing; 796221 (2011) https://doi.org/10.1117/12.878304
Event: SPIE Medical Imaging, 2011, Lake Buena Vista (Orlando), Florida, United States

Abstract

Optical microscopy poses many challenges for digital image analysis. One particular challenge includes correction of image artifacts due to respiratory motion from specimens imaged in vivo. We describe a non-rigid registration method using B-splines to correct these motion artifacts. Current attempts at non-rigid medical image registration have typically involved only a single pair of images. Extending these techniques to an entire series of images, possibly comprising hundreds of images, is presented in this paper. Our method involves creating a uniform grid of control points across each image in a stack. Each control point is manipulated by optimizing a cost function consisting of two parts: a term to determine image similarity, and a term to evaluate deformation grid smoothness. This process is repeated for all images in the stack. Analysis is evaluated using block motion estimation and other visualization techniques.

Citation Download Citation

Kevin S. Lorenz, Paul Salama, Kenneth W. Dunn, and Edward J. Delp "Non-rigid registration of multiphoton microscopy images using B-splines", Proc. SPIE 7962, Medical Imaging 2011: Image Processing, 796221 (11 March 2011); https://doi.org/10.1117/12.878304

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