research-article

The Capability of Beauveria Bassiana for Cellulase Enzyme Production

Authors:

Wanida Petlamul,

Thawatchai Sripornngam,

Narawadee Buakwan,

Kuntapon MahamadAuthors Info & Claims

ICBBB '17: Proceedings of the 7th International Conference on Bioscience, Biochemistry and Bioinformatics

Pages 62 - 66

https://doi.org/10.1145/3051166.3051167

Published: 21 January 2017 Publication History

Abstract

The objective of this study is to investigate the ability of cellulase enzyme production from Beauveria bassiana comparing Trichoderma viride (positive control) in the cellulose-agar medium using carboxymethylcellulose (CMC). The formation of clear zone by chromogenic reaction around the margin of the fungal colony demonstrated in CMC agar using agar diffusion method modified by congo -- red test. Both fungi were performed on cellulose-agar medium for 7 days at room temperature (30±2 °C). The result showed that clear zone number of B. bassiana was 5.0 mm higher than that of T. viride (4.7 mm). It revealed that B. bassiana had ability to release cellulase enzyme for cellulose degradation. Growth assessment of B. bassiana on CMC agar and potato dextrose agar (PDA) was carried out from radial growth and spore concentration after 3 - 15 days post inoculation. The growth of B. bassiana revealed that its radial growth collected from PDA (14.7-31.6 mm d-1) was higher than that of CMC agar (11.5-27.5 mm d-1). Its spore concentration on PDA was 2.4 × 108 spores mL-1 higher than that CMC (0.9 × 108 spores mL-1). Overall, the results of this study showed that B. bassiana had ability to produce cellulolytic enzymes on CMC agar. And its ability of cellulose degradation to be carbon source led to their growth.

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The Capability of Beauveria Bassiana for Cellulase Enzyme Production
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ICBBB '17: Proceedings of the 7th International Conference on Bioscience, Biochemistry and Bioinformatics

January 2017

72 pages

ISBN:9781450348324

DOI:10.1145/3051166

Copyright © 2017 ACM.

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Published: 21 January 2017

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ICBBB '17: 7th International Conference on Bioscience, Biochemistry and Bioinformatics

January 21 - 23, 2017

Bangkok, Thailand

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Djokić IKnežević ASavković ŽLjaljević Grbić MDimkić IBukvički DGavrilović DUnković N(2024)Characterization of Culturable Mycobiome of Newly Excavated Ancient Wooden Vessels from the Archeological Site of Viminacium, SerbiaJournal of Fungi10.3390/jof1005034310:5(343)Online publication date: 9-May-2024
https://doi.org/10.3390/jof10050343
Kadier AIlyas RHuzaifah MHarihastuti NSapuan SHarussani MAzlin MYuliasni RIbrahim RAtikah MWang JChandrasekhar KIslam MSharma SPunia SRajasekar AAsyraf MIshak M(2021)Use of Industrial Wastes as Sustainable Nutrient Sources for Bacterial Cellulose (BC) Production: Mechanism, Advances, and Future PerspectivesPolymers10.3390/polym1319336513:19(3365)Online publication date: 30-Sep-2021
https://doi.org/10.3390/polym13193365
Deb LDutta P(2021)Antagonistic potential of Beauveria bassiana (Balsamo) Vuillemin against Pythium myriotylum causing damping off of tomatoIndian Phytopathology10.1007/s42360-021-00372-wOnline publication date: 5-May-2021
https://doi.org/10.1007/s42360-021-00372-w
Hamrouni RDupuy NKarachurina AMitropoulou GKourkoutas YMolinet JMaiga YRoussos S(2021)Biotechnological potential of Zymotis‐2 bioreactor for the cultivation of filamentous fungiBiotechnology Journal10.1002/biot.20210028817:1Online publication date: 22-Oct-2021
https://doi.org/10.1002/biot.202100288
Amobonye ABhagwat PPandey ASingh SPillai S(2020) Biotechnological potential of Beauveria bassiana as a source of novel biocatalysts and metabolites Critical Reviews in Biotechnology10.1080/07388551.2020.1805403(1-16)Online publication date: 10-Aug-2020
https://doi.org/10.1080/07388551.2020.1805403

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