ABSTRACT
Electrochemical DNA sensor (E-DNA) have received attention in numerous application due to its high sensitivity, low cost, rapid response, simple, portability, and easy to operate. The objective of this study was to synthesis three Schiff bases derived from 4-amino-4'-nitrostilbene and 4-alkoxybenzaldehyde derivatives to be use as a potential linker in E-DNA. Schiff bases reaction are form from condensation reaction between primary amine and aldehyde at 79 °C using ethanol as solvent. All compounds are investigated and discussed by Fourier transform-infrared spectrometer (FTIR), UV-Vis spectrophotometer and Nuclear Magnetic Resonance (NMR). FTIR showed formation of C=N (imine) stretching vibrations at range 1604 cm-1 to 1608 cm-1. In UV-vis, absorbance of C=N (imine) group can be observed at peak range 385 nm to 388 nm. While in the 1H NMR the peak of CH=N (Imine) group was found at δH 8.34 ppm and 13C NMR for three Schiff bases were discovered at δC 158.80, 158.79 and 158.78 ppm.
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Index Terms
- Synthesis and Characterization of P-Nitro Stilbene Schiff Base as a Potential Linker in E-DNA
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