ABSTRACT
Serial-dilution method is used for estimating the concentration of unknown sample by counting the number of bacterial colonies cultured and then backtracking the measured counts to the unknown concentration. This process is time-consuming, error prone and cause eye fatigue. This paper proposes an automatic colony counter with captured image using webcam camera and uses Raspberry Pi for processing with capturing, thresholding, circular Hough transform, watershed segmentation and displaying results number of colonies on culture media.
- N. Otsu, "A Threshold Selection Method from Gray-Level Histograms," IEEE Transactions on Systems, Man, and Cybernetics, Vol. 9, No. 1, 1979, pp. 62-66.Google ScholarCross Ref
- Hüseyin Ateş, "An Image-Processing Based Automated Bacteria Colony Counter", Department of Electrical and Electronics Engineering, Anadolu University. 26555 Eskisehir Turkey, September 14-16, 2009.Google Scholar
- Shen Wei-zheng, Wu Ya-chun, Zhao lie, Zheng Hui, "Experimental Study for Automatic Colony Counting System Based on Image Processing", 2010 International Conference on Computer Application and System Modeling (ICCASM 2010).Google Scholar
- Ms. Hemlata Sethi, "Bacterial Colony Counter: Manual Vs Automatic", IRACST Engineering Science and Technology An International Journal (ESTIJ)Google Scholar
- Hong Men, Yujie Wu, Xiaoying Li, Zhen Kou, Shanrang Yang, "Counting Method of Heterotrophic Bacteria Based on Image Processing", IEEE 2008.Google Scholar
- Meyer, Fernand, "Topographic distance and watershed lines," Signal Processing, Vol. 38, July 1994, pp. 113-125. Google ScholarDigital Library
- Shih-Hsuan Chiu, Jiun-Jian Liaw, An effective voting method for circle detection, Pattern Recognition Letters, vol.26 no.2, pp.121-133. Google ScholarDigital Library
- J. M. Bewes, N. Suchowerska, D. R. Mckenzie "Automated cell colony counting and analysis using the circular Hough image transform algorithm (CHiTA)", Physics in Medicine and Biology 2008, vol. 53, no. 21, pp. 5991-6008.Google ScholarCross Ref
- Y.J. Zhang "A survey on evaluation methods for image segmentation", Pattern Recognition 29 (8) (1996) 1335 - 1340.Google ScholarCross Ref
Index Terms
- An Automated Colony Counter for Serial-Dilution Culture Method
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