ABSTRACT
Isolation of oil degrading strains from oil spill Beach Pseudomonas sp, Polyphenanthrene (PAHs) phenanthrene is used as a model for degradation research. The degradation of phenanthrene accords with first-order kinetics. When the concentration of phenanthrene is low, the degradation kinetics of both free and immobilized strains conformed to the linear simplified form of the classical Monod equation of biological treatment. When the concentration of phenanthrene is high, the degradation rate is greater than the maximum reaction rate of equation vmax. The semi-saturated constant Ks = 65.11 mg/L and the maximum reaction rate vmax = 17.42 d-1, for the degradation kinetics equation of free strains. The kinetic equation is: v=17.42S/65.11+S. Kinetic equation of calcium alginate immobilized strain: semi saturation constant: Ks = 60.02 mg/L Maximum reaction rate: vmax = 60.97 d-1. The kinetic equation is: v=60.97S/60.02+S. The degradation kinetics equation shows that the degradation efficiency of immobilized strains is much larger than that of free strains.
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Index Terms
- Microbial Degradation Kinetics of Polyphenanthrene Phenanthrene
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