Zhiwei Zhang
ABSTRACT
Electrical impedance flow cytometry (EIFC) based on microfluidic chip has been applied to the analysis of cells and micron particles, but the sensitivity needs further optimization for bacterial detections. In this paper, we propose an optimization of buffer conductivity to improve the sensitivity of EIFC. In this paper, polystyrene microspheres and yeast were suspended in buffers with different conductivity and detected by EIFC chip respectively. After analysing the signal-to-noise ratio (SNR) and coefficient of variation (CV) of the data, the optimum buffer conductivities for detection were obtained for Polystyrene microspheres (~1.6 S/m) and bacteria (~6.4 S/m), respectively. Then using this optimized microfluidic detection system, the Ø1.0 and Ø0.8 μm microspheres, Staphylococcus aureus and Staphylococcus albicans were analyzed, respectively. The results proved that the sensitivity of the EIFC chip can be improved by optimizing the buffer conductivity, which promotes a foundation for the detection, differentiation and analysis of submicron particles/bacteria in the future.
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Index Terms
- The Buffer Conductivity Optimization for Single Bacteria Detection Based on Electrical Impedance Flow Cytometry
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