AA Muhammad Nur Kasman
ABSTRACT
This study aims to evaluate the viability differences on oocyte vitrification surrounded by cumulus cells and oocyte vitrification not surrounded by cumulus cells after in vitro maturation. The oocyte collection was through the aspiration technique of puncturing the 10 cc disposable syringe with 18 G needle filled with oocyte medium on follicle 2-6mm. Oocytes with intact cytoplasm were chosen and divided into two treatment groups; oocytes surrounded by cumulus cells and not surrounded by cumulus cells. The oocyte vitrification used hemi-straw into liquid nitrogen for ten days and continued with warming. In vitro maturation, each was with 50 μl drop for 5-10 oocytes covered with mineral oil in incubator for 22 hours under environmental condition of 5% CO2 , temperature of 38 °C, and humidity of 95-99%. This study resulted in the morphological proportion of normal oocytes was 83.3% in the oocyte group surrounded by cumulus cells and 70% in the oocyte group not surrounded by cumulus cells. The oocyte viability on groups of oocyte surrounded by cumulus cells was insignificantly higher compared to the groups of oocyte not surrounded by cumulus cells after the vitrification and warming followed by in vitro maturation.
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