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Rapid multiplexed detection of cytokines with microfluidic chip

Published: 21 March 2021 Publication History

Abstract

We report an immunological method that integrates microfluidic chip and encoded beads to improve the accuracy and rapidity of molecules detection. The microfluidic chip for isolating individual beads in array can detect multiple cytokines simultaneously. The different kinds of encoded beads ware generated via two different polymers mixed at specific ratios. The surface of the beads was chemically modified to obtain sandwich-beads, making it capture specific cytokines. Two emission light were combined to excite encoded beads and anti-body simultaneously. Then we used CCD camera to take images. Finally, the images was analyzed by image processing algorithm to drew standard curves. We applied it to detect the concentration of IL-2, IL-10 and IL-6 cytokines in one sample simultaneously, and the results reach the level of pg/ml. The method used in this article for detecting the rare concentration of multi-cytokine raises the efficiency of immunology.

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        cover image ACM Other conferences
        BIC '21: Proceedings of the 2021 International Conference on Bioinformatics and Intelligent Computing
        January 2021
        445 pages
        ISBN:9781450390002
        DOI:10.1145/3448748
        Permission to make digital or hard copies of all or part of this work for personal or classroom use is granted without fee provided that copies are not made or distributed for profit or commercial advantage and that copies bear this notice and the full citation on the first page. Copyrights for components of this work owned by others than ACM must be honored. Abstracting with credit is permitted. To copy otherwise, or republish, to post on servers or to redistribute to lists, requires prior specific permission and/or a fee. Request permissions from [email protected]

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        • University of Arizona: University of Arizona

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        New York, NY, United States

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        Published: 21 March 2021

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        Author Tags

        1. cytokines
        2. encoded beads
        3. immunology
        4. microfluidic chip

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