Qiang Lin
ABSTRACT
We report an immunological method that integrates microfluidic chip and encoded beads to improve the accuracy and rapidity of molecules detection. The microfluidic chip for isolating individual beads in array can detect multiple cytokines simultaneously. The different kinds of encoded beads ware generated via two different polymers mixed at specific ratios. The surface of the beads was chemically modified to obtain sandwich-beads, making it capture specific cytokines. Two emission light were combined to excite encoded beads and anti-body simultaneously. Then we used CCD camera to take images. Finally, the images was analyzed by image processing algorithm to drew standard curves. We applied it to detect the concentration of IL-2, IL-10 and IL-6 cytokines in one sample simultaneously, and the results reach the level of pg/ml. The method used in this article for detecting the rare concentration of multi-cytokine raises the efficiency of immunology.
- Conlon, K.C, M.D. Miljkovic, and T.A. Waldmann. 2018. Cytokines in the Treatment of Cancer. J Interferon Cytokine Res. 39(1): p. 6--21. https://doi.org/10.1089/jir.2018.0019Google Scholar
Cross Ref
- Wang, X, et al. 2018. Cancer-associated fibroblasts induce epithelial-mesenchymal transition through secreted cytokines in endometrial cancer cells. Oncol Lett, 15(4): p. 5694--5702. https://doi.org/10.3892/ol.2018.8000Google ScholarCross Ref
- Baba, O.K., N.K. Sood, and K. Gupta. 2019. Clinical Evaluation of Glycoproteins and Inflammatory Cytokines in the Serum of Dogs Affected with Canine Mammary Cancer. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 89(4): p. 1465--1469. https://doi.org/10.1007/s40011-018-01073-7Google ScholarCross Ref
- Feng, L, et al. 2018. Serum levels of IL-6, IL-8, and IL-10 are indicators of prognosis in pancreatic cancer. J Int Med Res, 46(12): p. 5228--5236. https://doi.org/10.1177/0300060518800588Google ScholarCross Ref
- Lippitz, B.E. 2013. Cytokine patterns in patients with cancer: a systematic review. Lancet Oncol, 14(6): p. e218-e228. https://doi.org/10.1016/S1470-2045(12)70582-XGoogle ScholarCross Ref
- Vafajoo, A, et al. 2018. Early diagnosis of disease using microbead array technology: A review. Analytica Chimica Acta., 1032: p. 1--17. https://doi.org/10.1016/j.aca.2018.05.011Google ScholarCross Ref
- Wu, F, et al. 2016. The Role of Interleukin-17 in Lung Cancer. Mediators of Inflammation, 2016: p. 8494079. https://doi.org/10.1155/2016/8494079Google ScholarCross Ref
- Mi, D, W. Ren, and K. Yang. 2016. Adoptive immunotherapy with interleukin-2 & induced killer cells in non-small cell lung cancer: A systematic review & meta-analysis. Indian J Med Res, 143(Supplement): p. 1--10. https://doi.org/10.4103/0971-5916.191738Google ScholarCross Ref
- Julius, et al. 2017. Interleukin-10-regulated tumour tolerance in non-small cell lung cancer. British Journal of Cancer, 117: p. 1644--1655. https://doi.org/10.1038/bjc.2017.336Google ScholarCross Ref
- Siegel, R.L, et al. 2010. Cancer statistics, 2015. CA Cancer J Clin, 65(1): p. 5--29. https://doi.org/10.3322/caac.20073Google ScholarCross Ref
- Hecht and S. Stephen. 2008. Progress and Challenges in Selected Areas of Tobacco Carcinogenesis. Chemical Research in Toxicology, 21(1): p. 160--171. https://doi.org/10.1021/tx7002068Google ScholarCross Ref
- Ling, M.M, C. Ricks, and P. Lea. 2007. Multiplexing molecular diagnostics and immunoassays using emerging microarray technologies. Expert Review of Molecular Diagnostics, 7(1): p. 87--98. https://doi.org/10.1586/14737159.7.1.87Google ScholarCross Ref
- Robert, et al. 2006. Encoded Microcarriers For High-Throughput Multiplexed Detection. Angewandte Chemie International Edition, 45: p. 6104--6117. https://doi.org/10.1002/anie.200600288Google ScholarCross Ref
Index Terms
- Rapid multiplexed detection of cytokines with microfluidic chip
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