Yining Li
ABSTRACT
As one of the most diagnosed cancers in the world and the main cause for global cancer mortality, the treatment and prognosis of NSCLC is still a challenge. Some studies find that NF90, a double-stranded RNA binding protein, can regulate the expression of VEGF and further inhibit tumor angiogenesis and tumor growth in breast cancer and cervical cancer. In this article, experiments are designed to explore the correlation between NF90 and VEGF/HIF-1 in NSCLC through using siRNA to knock down NF90 in lung cancer cells and observing VEGF/HIF-1 expression by western blot and ELISA. Use Transwell assay and tube formation assay to detect the tube formation and cell migration of HUVECs in vitro while observe the tumor growth in vivo through Xenograft. Experiments also involve the exploration of PI3K/Akt/mTOR cellular pathway. The results supporting the hypothesis would indicate that NF90 is possibly a new anti-angiogenesis target for NSCLC treatment. And further study can explore the more specific mechanisms for the cooperation between NF90 and VEGF/HIF-1 and develop the novel targeted drugs for NSCLC.
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