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Regulation of FFA metabolism and attenuation of lipid accumulation through AMPK/SREBP-1c signaling by the extract of Apium graveolens L. root

Published: 11 July 2022 Publication History

Abstract

The extract of Apium graveolens L. root (AGRE) in Xinjiang has exhibited a lipid-lowering effects against development of hyperlipidemia in rat models. however, its underlying molecular mechanisms are largely unclear. The effects of AGRE and its major constituents were investigated on hepatic steatosis in L02 and HepG2 cells induced by free fatty acids (FFA). The results show that the contents of apigenin (AGN) and apiin (AIN) in AGRE were 11.69 μg/mg and 28.50 μg/mg, respectively. AGN, AIN and AGRE significantly reduced the content of triglyceride (TG) (P<0.05) and significantly decreased the levels of inflammatory factors interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) (P<0.05). The AGN, AIN and AGRE groups displayed elevated expression of adiponectin (ADPN) . At the protein level, AMP-activated protein kinase α (AMPKα ) α and acetyl-CoA carboxylase (ACC) phosphorylation increased (P<0.05), whilst sterol regulatory element-binding protein-1c (SREBP-1c) expression decreased (P<0.01). Therefore, AGRE and its major constituents have promising regulation effect of FFA metabolism and attenuation of lipid accumulation through AMPK/SREBP-1c signaling.

References

[1]
Boutari C, Perakakis N, Mantzoros C S 2018 Endocrinol. Metab. 33 33-43
[2]
Hardie DG Acta. Pharm. Sin. B. 6 1-19
[3]
Yang M, Li X, Zeng X, Ou Z, Xue M, Gao D, Liu S, Li X and Yang S 2016 Am. J. Chinese Med. 44 551-64
[4]
Schindler M, Pendzialek M, Grybel K J, Seeling T, Gürke J, Fischer B and Navarrete S A 2017 Hum. Reprod. 32 1382-92.
[5]
Ferre P, Phan F and Foufelle F 2021 Biochem. J. 478 3723-39
[6]
Kohjima M, Higuchi N, Kato M, Kotoh K, Yoshimoto T, Fujino T, Yada M, Yada R, Harada N and Enjoji M 2008 Int. J. Mol. Med. 21 507-11
[7]
Chen L Y, Duan Y Q, Wei H Q, Ning H X, Bi C F, Zhao Y, Qin Y and Li Y L 2019 Expert. Opin. Inv. Drug 28 917-30.
[8]
Bessone F, Razori M V and Roma M G 2019 Cell Mol. Life Sci. 76 99-128.
[9]
Abenavoli L, Boccutoto L, Masarone M, Pellicano R and Persico M 2018 Minerva Biotecnol, 30, 36-40.
[10]
Chen S L and Lin Y 2006 Quintessence of Materia Medica (Beijing: Military Medical Science Press) p 318.
[11]
Tian L, He Y, Li Y and Wu L Y 2019 J. Xin jiang Med. Univ. 42 988-93.
[12]
He Y, Shi Y, Zhang A R, Zhang X X, Sun J and Tian L 2019 RSC Adv. 46 26757-67
[13]
Xie C, Chen Z, Zhang C, Xu X, Jin J, Zhan W, Han T and Wang J 2016 Life Sci. 157 131-9.

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ICBET '22: Proceedings of the 12th International Conference on Biomedical Engineering and Technology
April 2022
237 pages
ISBN:9781450395779
DOI:10.1145/3535694
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Association for Computing Machinery

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Publication History

Published: 11 July 2022

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Author Tags

  1. AMPK
  2. Apium graveolens L. root
  3. SREBP-1c
  4. hepatic steatosis

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  • Research-article
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  • Refereed limited

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  • National Natural Science Foundation of China

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ICBET 2022

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