Xianghua Hou
ABSTRACT
Bromelain, a well-known cysteine-protease found in pineapple plant, is able to be applied as oral drug for the treatment of certain diseases such as inflammation. However, most enzymes are fragile and even small structural changes may reduce their activity. As reported previously, the stability of enzymes can be greatly enhanced by utilizing nanoparticles. Therefore, the investigation of the interaction between bromelain and nanoparticle is very interesting and also of significance for drug delivery and other related fields. In this work, the regenerated cellulose acetate microspheres were prepared by the reverse suspension method, and their adsorption characteristics for bromelain were researched. Experimental results indicated that the cellulose acetate microspheres introduced with carboxyl groups possess a larger specific surface area and excellent adsorption capacity. Moreover, effect of incubation time, temperature, initial concentration of bromelain and medium pH on adsorption behavior was studied and it was found that maximum bromelain loading on the cellulose acetate microspheres was obtained when adsorption process was implemented at pH 9.0, 40 °C for 240 min with 1400 mg/L bromelain. This work may provide some new insights into the effective application of cellulose acetate microspheres in biomedical fields.
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