The strain distribution of medical elastic culture membrane is measured by multi-cameras digital image correlation (DIC) technique under the effects of different mechanical stretching. The objective of this work is to provide basis for observing cell morphology, cell production, and apoptosis according to the strain distribution on the elastic membrane surface. The multi-cameras DIC system is used for the first time to measure the full-field strain of the medical elastic membrane induced by the different size of indenters. Therefore, the curves of strain distribution for elastic membrane with the increasing depth of indenters are also given as well. And the depth of field in the DIC system is also discussed. The results from serials of experiments for four indenters can be obtained that the strain distribution along the radius from center to fixed boundaries is firstly smaller, and then becomes bigger and following the strain becomes smaller again. In addition, the strain distribution is changed with the increasing depths of indenter impressed into the elastic membrane. At the first stage, the strain values in the center of elastic membrane are bigger than other parts, however, with the increasing depths of indenter impressed into the elastic membrane, the maximum strain values occur in a certain part between the center and fixed boundaries of elastic membrane. Through studying the strain distribution of elastic membrane under effects of different tension force, the full-field large strain on the elastic membrane is successfully obtained. The mechanism of remodeling and responding to the mechanical tension force for individual cells can be well understood.