skip to main content
10.1145/3543377.3543380acmotherconferencesArticle/Chapter ViewAbstractPublication PagesicbbtConference Proceedingsconference-collections
research-article

An autofocus algorithm for microscopic hyperspectral imaging system with adaptive wavelength variation

Published: 08 August 2022 Publication History

Abstract

Microscopic hyperspectral imaging techniques have been widely used to analyze information from digital pathology sections. Real-time autofocusing is one of the important techniques to acquire high-quality images efficiently; however, there are few autofocusing algorithms are proposed in this field. Therefore, this article proposes an autofocus algorithm for a self-developed microscopic hyperspectral imaging system. This method can finish autofocus with a single field of view in 0.9 seconds. In addition, to reduce the focusing time, we fit a wavelength as a function of focal length. We applied the above method on our own dataset and the experimental results show that this method can acquire large-scale microscopic hyperspectral images quickly and accurately.

References

[1]
U. A. Bhatti, "Local Similarity-Based Spatial-Spectral Fusion Hyperspectral Image Classification With Deep CNN and Gabor Filtering," Ieee Transactions on Geoscience and Remote Sensing, vol. 60, 2022.
[2]
L. I. Qingli, X. Gonghai, X. U. E. Yongqi, and Z. Jingfa, "Microscopic Hyperspectral Image Study of Human Blood Cells," Opto-Electronic Engineering, vol. 35, no. 5, pp. 98-101, 2008.
[3]
Q. Wang, "Identification of Melanoma From Hyperspectral Pathology Image Using 3D Convolutional Networks," Ieee Transactions on Medical Imaging, vol. 40, no. 1, pp. 218-227, Jan 2021.
[4]
Q. Wang, J. Wang, M. Zhou, Q. Li, Y. Wen, and J. Chu, "A 3D attention networks for classification of white blood cells from microscopy hyperspectral images," Optics and Laser Technology, vol. 139, Jul 2021.
[5]
Q. Zhang, Y. Wang, S. Qiu, J. Chen, L. Sun, and Q. Li, "3D-PulCNN: Pulmonary cancer classification from hyperspectral images using convolution combination unit based CNN," Journal of Biophotonics, vol. 14, no. 12, Dec 2021.
[6]
C.-C. Gu, K.-J. Wu, J. Hu, C. Hao, and X.-P. Guan, "Region Sampling for Robust and Rapid Autofocus in Microscope," Microscopy Research and Technique, vol. 78, no. 5, pp. 382-390, May 2015.
[7]
W. Wang, Z. Zheng, and H. Shen, "A Fast Autofocus Method for Multispectral Imaging System," Opto-Electronic Engineering, vol. 40, no. 9, pp. 35-40,45, 2013 2013,.
[8]
Y. Wang, H. Feng, Z. Xu, Q. Li, and Y. Chen, "Autofocus Evaluation Function Based on Saturate Pixels Removing," Acta Optica Sinica, vol. 36, no. 12, pp. 1210001-1-1210001-8, 2016.
[9]
H. Zhao, Y. Shen, and M. Huang, "Improvement of the Microscope Autofocus Range Based on the Spectrum Analysis Method," Opto-Electronic Engineering, vol. 40, no. 6, pp. 78-83, 2013.
[10]
S. Chowdhury, M. Kandhavelu, O. Yli-Harja, and A. S. Ribeiro, "An interacting multiple model filter-based autofocus strategy for confocal time-lapse microscopy," Journal of Microscopy, vol. 245, no. 3, pp. 265-275, Mar 2012.
[11]
Y. Liu and L. Jing, "Contrast optimization autofocus algorithm," Journal of electronics & information technology, vol. 25, no. 1, pp. 24-30, 2003.
[12]
G.-h. Xiao, R. Shu, and Y.-q. Xue, "Design of microscopic hyperspectral imaging system," Optics and Precision Engineering, vol. 12, no. 4, pp. 367-72, Aug. 2004.
[13]
L. Fang, C. Wang, S. Li, and J. A. Benediktsson, "Hyperspectral Image Classification via Multiple-Feature-Based Adaptive Sparse Representation," Ieee Transactions on Instrumentation and Measurement, vol. 66, no. 7, pp. 1646-1657, Jul 2017.
[14]
Z. He, Q. Wang, Y. Shen, J. Jin, and Y. Wang, "Multivariate Gray Model-Based BEMD for Hyperspectral Image Classification," Ieee Transactions on Instrumentation and Measurement, vol. 62, no. 5, pp. 889-904, May 2013.
[15]
Q. Zhang, "An autofocus algorithm considering wavelength changes for large scale microscopic hyperspectral pathological imaging system," Journal of Biophotonics, 2022.
  1. An autofocus algorithm for microscopic hyperspectral imaging system with adaptive wavelength variation

    Recommendations

    Comments

    Information & Contributors

    Information

    Published In

    cover image ACM Other conferences
    ICBBT '22: Proceedings of the 14th International Conference on Bioinformatics and Biomedical Technology
    May 2022
    190 pages
    ISBN:9781450396387
    DOI:10.1145/3543377
    Permission to make digital or hard copies of all or part of this work for personal or classroom use is granted without fee provided that copies are not made or distributed for profit or commercial advantage and that copies bear this notice and the full citation on the first page. Copyrights for components of this work owned by others than ACM must be honored. Abstracting with credit is permitted. To copy otherwise, or republish, to post on servers or to redistribute to lists, requires prior specific permission and/or a fee. Request permissions from [email protected]

    Publisher

    Association for Computing Machinery

    New York, NY, United States

    Publication History

    Published: 08 August 2022

    Permissions

    Request permissions for this article.

    Check for updates

    Author Tags

    1. Autofocus
    2. Large scale imaging
    3. Microscopic hyperspectral imaging
    4. Pathology
    5. Self-adaptive focusing

    Qualifiers

    • Research-article
    • Research
    • Refereed limited

    Funding Sources

    Conference

    ICBBT 2022

    Contributors

    Other Metrics

    Bibliometrics & Citations

    Bibliometrics

    Article Metrics

    • 0
      Total Citations
    • 80
      Total Downloads
    • Downloads (Last 12 months)25
    • Downloads (Last 6 weeks)0
    Reflects downloads up to 05 Mar 2025

    Other Metrics

    Citations

    View Options

    Login options

    View options

    PDF

    View or Download as a PDF file.

    PDF

    eReader

    View online with eReader.

    eReader

    HTML Format

    View this article in HTML Format.

    HTML Format

    Figures

    Tables

    Media

    Share

    Share

    Share this Publication link

    Share on social media