Molecular Identification and Characterization of Vitamin D Receptor in Grass Carp (Ctenopharyngodon idella)
Authors: 
Hao Sun, Pei Ge, Yueyue Wang, Qingqing Liu, Hong ZhouAuthors Info & Claims
Hao Sun, Pei Ge, Yueyue Wang, Qingqing Liu, Hong ZhouAuthors Info & ClaimsPages 102 - 107
Abstract
In the present study, two vitamin D receptor (VDR) sequences were obtained in grass carp and named them as gcVDRa and gcVDRb, respectively. The gcVDRa consisted of 1359 base pairs and encoded 452 amino acids with two conserved domains (Ligan binding domain, LBD and DNA binding domain, DBD). The gcVDRb had 1269 base pairs and encoded 422 amino acids with a LBD not DBD. Given that gcVDRb lacked the DBD, here we especially concerned the characterization of gcVDRa. Multiple sequence alignment analysis showed that gcVDRa shared 79.3% identities with the orthologues of human, mouse, snake and zebrafish. Additionally, the 3D structure of gcVDRa was established by using homology modeling method, founding that gcVDRa had a zinc finger structure. Furthermore, gcVDRa was overexpressed in EPC cells, and then was found to translocate into the nucleus induced by 1,25D3. In conclusion, our study revealed that there are two VDR isoforms in grass carp and gcVDRa is likely to function as a transcription factor as seen in mammals.
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- Molecular Identification and Characterization of Vitamin D Receptor in Grass Carp (Ctenopharyngodon idella)
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Published In
May 2022
190 pages
ISBN:9781450396387
DOI:10.1145/3543377
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Published: 08 August 2022
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- The Sichuan Science and Technology Program, China
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ICBBT 2022
ICBBT 2022: 2022 14th International Conference on Bioinformatics and Biomedical Technology
May 27 - 29, 2022
Xi'an, China
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